WASP is an application that designs allele-specific (AS) primers for detecting SNPs and mutations. WASP input can be either key searches (Section A) or SNPs with flanking sequences (Section B). Users can input key searches (SNP IDs or Gene Names), consequently, WASP returns the search result as a graphical interface including SNP information, AS primers (if exist) and PCR condition. SNPs with flanking sequences can be input to the system via the text box or the file uploading module. Additionally, users are able to adjust some primer picking parameters based on the desired PCR condition (Section C).
Genome Institute, BIOTEC
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BMC Genomics. 2007 Aug 14;8:275.
WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations.
Wangkumhang P1, Chaichoompu K, Ngamphiw C, Ruangrit U, Chanprasert J, Assawamakin A, Tongsima S.