UniFrag / GenomePrimer
UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.
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Sacha A. F. T. van Hijum, Anne de Jong, Girbe Buist, Jan Kok and Oscar P. Kuipers
UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons
Bioinformatics (2003) 19 (12): 1580-1582.