Trace Recalling 0.5
Trace Recalling is a novel method for deconvoluting double traces that result from simultaneously sequencing two DNA templates. Trace Recalling identifies up to two bases at each position of such a trace. The resulting ambiguity sequence is aligned to the genome, identifying one template sequence. A second template sequence is then inferred from this alignment. This technique makes possible many exciting biological applications.
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Tenney A.E., Wu J.Q., Langton L., Klueh P., Quatrano R., Brent M.R.. 2007.
“A tale of two templates: automatically resolving double traces has many applications, including efficient PCR-based elucidation of alternative splices.”
Genome Research 17(2):212-218.