SpotOn – Determination of Hot-Spots at Protein-protein interfaces

SpotOn

:: DESCRIPTION

SpotOn is a robust algorithm developed to identify and classify the interfacial residues as Hot-Spots (HS) and Null-Spots (NS) with a final accuracy of 0.95 and a sensitivity of 0.95 on an independent test set.

::DEVELOPER

BONVIN LAB

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser

:: DOWNLOAD

 NO

:: MORE INFORMATION

Citation

SpotOn: High Accuracy Identification of Protein-Protein Interface Hot-Spots.
Moreira IS, et al.
Sci Rep, 7 (1), 8007 2017 Aug 14

HotSpot Wizard 3.0 – Identification of Hot Spots in Protein Engineering

HotSpot Wizard 3.0

:: DESCRIPTION

HotSpot Wizard is a web server for automatic identification of ‘hot spots’ for engineering of substrate specificity, activity or enantioselectivity of enzymes and for annotation of protein structures.

::DEVELOPER

Protein Engineering Group, Loschmidt Laboratories

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser

:: DOWNLOAD

 NO

 :: MORE INFORMATION

Citation

HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information.
Sumbalova L, Stourac J, Martinek T, Bednar D, Damborsky J.
Nucleic Acids Res. 2018 Jul 2;46(W1):W356-W362. doi: 10.1093/nar/gky417.

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering.
Bendl J, Stourac J, Sebestova E, Vavra O, Musil M, Brezovsky J, Damborsky J.
Nucleic Acids Res. 2016 May 12. pii: gkw416.

Nucleic Acids Res. 2009 Jul;37(Web Server issue):W376-83. doi: 10.1093/nar/gkp410. Epub 2009 May 21.
HotSpot Wizard: a web server for identification of hot spots in protein engineering.
Pavelka A1, Chovancova E, Damborsky J.

AGGRESCAN – Prediction and Evaluation of “hot spots” of Aggregation in Polypeptides

AGGRESCAN

:: DESCRIPTION

AGGRESCAN is a web-based software for the prediction of aggregation-prone segments in protein sequences, the analysis of the effect of mutations on protein aggregation propensities and the comparison of the aggregation properties of different proteins or protein sets.

::DEVELOPER

Xavier Daura

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser

:: DOWNLOAD

 NO

:: MORE INFORMATION

Citation:

BMC Bioinformatics. 2007 Feb 27;8:65.
AGGRESCAN: a server for the prediction and evaluation of “hot spots” of aggregation in polypeptides.
Conchillo-Solé O1, de Groot NS, Avilés FX, Vendrell J, Daura X, Ventura S.