siDirect is a web server for providing efficient and target-specific siRNA design for mammalian RNAi. In this new version, the siRNA design algorithm has been extensively updated to eliminate off-target silencing effects by reflecting our recent finding that the capability of siRNA to induce off-target effect is highly correlated to the thermodynamic stability of the ‘seed’ duplex.
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siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect.
Naito Y, Yoshimura J, Morishita S, Ui-Tei K.
BMC Bioinformatics. 2009 Nov 30;10:392. doi: 10.1186/1471-2105-10-392.