RITA (Rapid Identification of Taxonomic Assignments) is a standalone software package and Web server for taxonomic assignment of metagenomic sequence reads. By combining homology predictions from BLAST or UBLAST with compositional classifications from a Naive Bayes classifier, RITA is able to achieve very high accuracy on short reads. Unlike other hybrid approaches which combine these predictions for all sequences to be classified, RITA uses a pipeline to first identify cases where both types of classifier are in agreement, which constitute the highest-confidence set. Sequences not classified in this manner are subjected to a series of downstream classification steps.
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MacDonald NJ, Parks DH, and Beiko RG.
Rapid identification of high-confidence taxonomic assignments for metagenomic data
Nucl. Acids Res. (2012) doi: 10.1093/nar/gks335 April 4, 2012.