REPK (Restriction Endonuclease Picker) finds sets of 4 commercially available restriction endonucleases which together uniquely differentiate designated sequence groups from a supplied FASTA format sequence file for use in T-RFLP. If you have a database of known sequences from an environment, this program could be useful to pick some enzymes that uniquely discriminate the different groups in your database.
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Collins, R. E. and G. Rocap. 2007.
REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis.
Nucleic Acids Res. 35 (Database issue): W58-W62; doi:10.1093/nar/gkm384