Refcomp was designed to analyze sequencing traces which contains data from strictly homozygous samples (eg. cloned DNA, mitochondrial DNA, etc.). This data represents a special case which can be analyzed for mismatches with a known reference sequence. Refcomp will determine the high quality positions within an assembled DNA contig and produce a report listing sites which differ from a defined reference sequence.
Refcomp is designed as a member of an integrated suite of sequence analysis applications which includes Phred,Phrap and Consed, and is not a stand alone program.
Dr. Deborah Nickerson’s lab at the University of Washington
- Linux/Mac OsX/Solaris/SGI IRIX/Compaq Tru64 Alpha/HP-UX
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RefComp is available for free to researchers at academic and non-profit institutions. To aquire RefComp, please Read the Academic License Agreement, and fill in and submit the request form.
Rieder et al,Automating the identification of DNA variations using quality-based fluorescence re-sequencing: analysis of the human mitochondrial genome., Nucleic Acids Research, 26: 967-973, 1998.