QPCR is a versatile web-based Java application that allows to store, manage, and analyze data from quantitative real-time polymerase chain reaction (qPCR) experiments. It comprises a parser to automatically import generated data from qPCR devices and includes a variety of analysis methods to calculate Ct and amplification efficiency values. The analysis pipeline includes technical and biological replicate handling, incorporates sample or gene specific efficiency, normalization using single or multiple housekeeping genes, inter-run calibration, and fold change calculation. Moreover, the application supports proper error propagation throughout all analysis steps and allows conducting statistical tests on sample classes. Results can be visualized in highly customizable charts and exported for further investigation.
Genomics & Bioinformatics Graz, Graz University of Technology
- Linux / Windows / MacOsX
- Oracle / PostgreSQL / MySQL
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Pabinger S, Thallinger GG, Snajder R, Eichhorn H, Rader R, Trajanoski Z.
QPCR: Application for real-time PCR data management and analysis.
BMC Bioinformatics 2009, 10:268