Primer3 2.3.6 / Primer3-web 4.0.0 / Primer3Plus 2.3.6 – PCR Primer Design Tool

Primer3 2.3.6 / Primer3-web 4.0.0 / Primer3Plus 2.3.6


Primer3 is a widely used program for designing PCR primers (PCR = “Polymerase Chain Reaction”). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.

Primer3-web is the original web-interface to Primer3.

Primer3Plus is an updated, task-oriented web-interface to Primer3.

Primer3 Web Online Version

Primer3 Plus Online Version


developed at Whitehead_Institute and Howard Hughes Medical Institute by Steve Rozen and Helen Skaletsky.



  • Linux / Windows / Mac OsX
  • perl





Steve Rozen and Helen J. Skaletsky (2000)
Primer3 on the WWW for general users and for biologist programmers.
In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp 365-386

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