PIUS is an open source tool that performs peptide identification from tandem mass spectrograms using the six-frame translation of the complete genome. Differently from peptidomics methods that also allow a search against the complete genome, PIUS does not limit analysis of the genome to a small set of sequences that match a list of de novo reconstructions. Instead, it performs an exhaustive scan of the translation of the six reading frames of the complete genome. Therefore, this search is not biased towards a subset of candidates.
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Bioinformatics. 2013 Aug 1;29(15):1913-4. doi: 10.1093/bioinformatics/btt298. Epub 2013 May 24.
PIUS: peptide identification by unbiased search.
Costa EP1, Menschaert G, Luyten W, De Grave K, Ramon J.