PCR / Primer Design
- Primer Wizard – Speed up the Cloning of Coding Regions of a Gene via PCR method
- MPRIMER 20121218 – High-throughput Multiplex PCR Primer Design
- Primer3 2.3.5 / Primer3-web 4.0.0 / Primer3Plus 1.1.0 – PCR Primer Design Tool
- LinRegPCR 2013.03.28 – Analysis of Quantitative PCR Data
- FastPCR 6.3.42 – PCR Primers or Probe Design
- PD5 1.3 – Software Library for the Development of Primer Design Applications
- AmplifX 1.6.1 – Manage & Design Primers for PCR
- AlleleID 7.80 – Assay Design for Bacterial Identification
- AmpliconNoise 1.28 – Noise Removal from Pyrosequenced Amplicons
- QUMA 1.1.12 – Visualize and Quantify Bisulfite Sequence data for CpG Methylation Analysis
- UniPrime2 2.10 – Universal Primer Design Server
- Q-Gene 1.2 – Process Quantitative Real-time RT-PCR Data
- In-Silico PCR 34 – Searches a Sequence Database with a pair of PCR Primers
- jPCR 1.15.8 – Java Tools for PCR, in silico PCR, Oligonucleotide Assembly & Analysis
- e-PCR 2.3.12 – Search DNA Sequences for Sequence Tagged Sites
- qbasePLUS 2.0 – Real-time PCR Data Analysis
- PrimerPlex 2.62 – Oligo Design for Multiplex PCR
- NoePrimer 3.0 – Primer Design Software
- Oligo 7.57 – Primer Analysis Software
- dnaMATE 1.0 – Consensus Melting Temperature Prediction server for short DNA sequences
- PROBER 2.1 – Fish Oligo Probe Design Software
- Easy PCR 1.03 – App for people working with PCR
- PCR MasterMix 1.0 – Let the MasterMix get easier and quicker
- Bio-Rad Real-Time PCR iPhone Application 2.0
- MIQE qPCR 1.3 – Check qPCR Experiments
- Real-Time PCR 1.7 – Real-time PCR resources for Miblie Phone
- CloningBench 1.0 – Guide of Cloning Experiments for iPhone / iPAD
- BiSearch 2.32 – Primer Design and Search Tool
- UniFrag / GenomePrimer 1.1 – High-throughput Primer Design software
- PriSM – Primer Selection and Matching for Amplification of Viral Genomes
- PrimerPy 0.97a – GUI tool for QPCR Primer Design
- OligoFaktory SE – Oligonucleotide Design
- Mutagenesis Primer Generator – Generate Mutagenesis Primer
- ConservedPrimers 2.0 – High-throughput pipeline for Intron-flanking PCR Primer Design
- BatchPrimer3 1.0 – High Throughput Web Application for PCR and Sequencing Primer Design
- RJPrimers 1.0 – Transposable Element Junction based PCR Primer Design
- ProbeMaker 1.4.1 – Design and Analysis of sets of Oligonucleotide Probes
- Disperse 1.0.3 – Design of Selector Assays for Targeted Resequencing Applications
- DEODAS 0.2.0 -DEgenerate Oligo Nucleotide Design & Analysis System
- GenomeMasker 1.3 – Design Unique Genomic PCR Primers
- mPrimer3 1.0 – Modification of Primer3
- MultiPLX 2.1 – Analyze PCR Primer Compatibility
- SLICSel 1.1 – Design Specific Oligonucleotide Probes for Microbial Detection and Identification
- HTqPCR 1.13.0 – Analysis of High-throughput Quantitative Real-time PCR data
- Beacon Designer 8.02 – Automate Real Time PCR Primer & Probe Design
- Primer Premier 6.12 – PCR Primer Design Software
- StarPrimer D’Signer 3.0.0.3 – Design of Primers for ENTRY Cloning to Create Donor Vector
- Primer D’Signer 1.1 – Primer Designer for pASK-IBA, pEXPR-IBA & pPR-IBA Vectors
- LAMP Designer 1.02 – Design Primers for Loop-Mediated Isothermal Amplification Assays
- Array Designer 4.30 – Oligo & cDNA Microarray Design Software
- QPCR 1.0 – Store, Manage, and Analyze data from qPCR Experiments
- Oligo Heat Map 1.51 – Check Thermodynamical Parameters for PCR primers
- Assembly PCR Oligo Maker – Design Oligodeoxynucleotides For Constructing Long DNA Molecules
- Amplicon b09 – Software for Designing PCR Primers on Aligned DNA Sequences
- PhiSiGns 1.2 – Indentify Signature Genes in Phages and Design Primers
- FindProbe v2 – Design Probes for Microarrays
- Primer Factory 2.0 – Tool for Searching and Designing Primers
- Pythia 1.0.1 – Thermodynamically based PCR primer design program
- pcrcoal 1.0 – Coalescent Simulations of PCR
- PCR Analyzer v 1.02 – Estimate for Initial Amount of Amplicon Present in a PCR
- Primegens 2.0.5 – Primer Design for Microarray
- HYDEN – A software for Designing Degenerate Primers
- ProbeSpec – Map Specificity of all Candidate Probes for a Given Sequence
- QDD 2.1 – Select Microsatellite Markers and Design Primers
- Realyser 1.0 – Real Time PCR Normalisation tool
- qcalculator 1.0 – Calculate Relative mRNA Gene Expression
- qPCR-DAMS 1.2 – Analyze, Manage, and Store Quantitative Real-Time PCR data.
- PerlPrimer 1.1.21 – Open-source PCR Primer Design
- NormFinder 0.953 – Normalization of Real-Time Quantitative Reverse Transcription-PCR Data
- DART-PCR 1.0 – Quantitative Real-time PCR Data Analysis
- GenEx 5.3.6 – Analyze Real-time qPCR Data
- OligoCalc 3.19 – Oligonucleotide Properties Calculator
- primerD 1.0 – Design of Unique Degenerate Primer Pairs
- MaxAlike 1.0 – Phylogenetic Reconstruction of incomplete Sequences by Maximum Likelihood
- MPprimer 1.4 – Reliable Multiplex PCR Primer Design
- MFEprimer 2.0 – PCR Primer Specificity Checking Program
- GenoFrag 2.1 – Design Primers Optimized for Whole Genome Scanning by Long-range PCR Amplification
- IThOS 2.1 – Tool for Designing Oligonucleotide Primers
- Methyl Primer Express 1.0 – Design PCR Primers for Methylation Mapping Experiments
- Primer Prim’er 5.6.0 – PCR Primer Design Tool
- ORFprimer 1.6.4.1 – High Throughput PCR Primer Design
- AutoDimer 1.0 – Screening Tool for Primer-dimer & Hairpin Structures
- PCRsetup – Tool for Planning PCR and qPCR Reactions
- NetPrimer – Free Primer Analysis Software
- MeltCalc 2.0 – Thermodynamic Probe Design
- Xpression Primer 3.03 – Primer Design tool for Expression Cloning Experiments
- PrimerHunter 1.0.2 – Primer Design for PCR-Based Virus Subtype Identification
- G-POT 1.0 / PRIMER-ILP 1.0 – Multiplex-PCR Primer Set Selection
- DNA-BAR – Select DNA Probes
- SeqState 1.4.1 – Primer Design, Sequence Statistics & Indel Coding
- TGGE-STAR – Facilitate the Design of PCR Primers
- JCVI Primer Designer 20101122 – High Throughput PCR Primer Design


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