FLICK 1.1 – PCL File Linker

FLICK 1.1

:: DESCRIPTION

FLICK takes multiple PCL files and creates a single aggregate PCL file.FLICK is written in perl.

Intended for joining datasets which have been created on separate occassions, and which may have different ID values (normally requiring the creation of a database to join the data fields).

::DEVELOPER

Falkow Lab

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / Mac Os / Linux
  • Perl
  • TK

:: DOWNLOAD

FLICK for WinSource Code

:: MORE INFORMATION

The software is copyrighted under the terms of the GNU General Public License. You can view this license at http://www.gnu.org/licenses/gpl.txt.

CCACK 1.0 – Constant Cutoff Analysis

CCACK 1.0

:: DESCRIPTION

CCACK takes an input PCL or CDT (pre- or post-clustering) file and converts all values to a binary scale. CCACK is written in perl. The cutoff is user-defined. Primarily intended for genomotyping analysis. Also see GACK below.

::DEVELOPER

Falkow Lab

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / Mac Os / Linux
  • Perl
  • TK

:: DOWNLOAD

CCACK for Win ; Source Code

:: MORE INFORMATION

The software is copyrighted under the terms of the GNU General Public License. You can view this license at http://www.gnu.org/licenses/gpl.txt.

AACK 1.0 – Add Annotation

AACK 1.0

:: DESCRIPTION

AACK adds annotation from a file to a PCL (pre-clustering) data file. AACK is written in perl.

The annotation file requires an ID and Name column. The ID’s are read from the first column of the PCL file, and the annotations are added to the second column. Does not affect the data. Intended for situations in which a different annotation is desired, such as when an annotation is published for a microarray constructed pre-annotation.

::DEVELOPER

Falkow Lab

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / Mac Os / Linux
  • Perl
  • TK

:: DOWNLOAD

AACK for Win ;  Source Code

:: MORE INFORMATION

The software is copyrighted under the terms of the GNU General Public License. You can view this license at http://www.gnu.org/licenses/gpl.txt.

NIA Array Analysis Tool 2.0 – Statistical Significance Analysis of Microarray Data

NIA Array Analysis Tool 2.0

:: DESCRIPTION

NIA Array Analysis Tool is designed to test statistical significance of gene microarray data, visualize the results, and provide links to clone information and gene index.

  • Evaluate the statistical significance of differential gene expression based on microarray data
  • Make log-ratio plots and scatter-plots
  • Find clusters of tissues with similar expression patterns and identify specific genes for each cluster
  • Find major patterns of variability in gene expression using Principal Component Analysis (PCA) and biplot
  • Cluster genes according to their contribution to principal components
  • Find genes whose expression matches a given pattern (pattern matching)
  • Plot the dendrogram for replications to check abnormal arrays
  • Plot the error function (SD vs. expression level)
  • Make a correlation matrix
  • Normalize input data
  • Import principal components from an earlier analysis to overlay 2 sets of results
  • Save results of analysis for personal or public access

Arrayjoin Tool is designed for compiling an input file from multiple scanner files.

ANOVA Tool – Statistical analysis is based on the single-factor ANalysis Of VAriance

Hierarchical clustering of tissues is done using the average distance method.

Principal component analysis (PCA) is done using the Singular Value Decomposition (SVD) method that generates eigenvectors both for rows and columns of the log-transformed data matrix (Gabriel 1971.

Pattern matching can be used to find genes with expression pattern similar to some other gene (or group of genes).

Gene list analysis – Getting information on selected genes is one of most important components of microarray analysis.

::DEVELOPER

Laboratory of Genetics, National Institute on Aging,  NIH

:: SCREENSHOTS

:: REQUIREMENTS

:: DOWNLOAD

NIA Array Analysis Tool

:: MORE INFORMATION

Reference:
Sharov, A.A., Dudekula, D.B., Ko, M.S.H. 2005. A web-based tool for principal component and significance analysis of microarray data. Bioinformatics, 21(10): 2548-9. Epub 2005 Feb 25. PMID: 15734774

GAAS 1.0.0.1 – Gene Array Analyzer Software

GAAS 1.0.0.1

:: DESCRIPTION

GAAS (Gene Array Analyzer Software) is an integrated software framework for efficient management, analysis and visualization of large amounts of gene expression data across replicated experiments. It is structured in management, analysis and visualization sections that allow dealing with several gene expression dataset formats, custom differential expression data analyses, suitable visualization, and storage of results.

The software performs pre-processing of gene expression data transforming any input data structure in MS-Excel format into a built-in database-based data structure in MS-Access format, perform fast differential gene expression analyses across multiple replica experiments.

GAAS is designed for a multi-user environment, enabling each user to store its own parameter values used to perform the analyses, and define data visualization schema and format of the output data.

::DEVELOPER

Pietro Cerveri, PhD  &  Marco Masseroli, PhD

:: SCREENSHOTS

:: REQUIREMENTS

:: DOWNLOAD

GAAS ; Testing Data Packages

:: MORE INFORMATION

Citation:

Masseroli M, Cerveri P, Pelicci PG, Alcalay M.
GAAS: Gene Array Analyzer Software for management, analysis and visualization of gene expression data.
Bioinformatics 2003 April 12; 19(6): 774-775.
[Abstract], [PDF], [PubMed].

CAGED 1.1 – Cluster Analysis of Gene Expression Dynamics

CAGED 1.1

:: DESCRIPTION

CAGED (Cluster Analysis of Gene Expression Dynamics) is a model based,Bayesian clustering procedure developed by Ramoni et al. to cluster gene expression profiles measured with microarrays in temporal experiments. Contrary to popular clustering methods, CAGED takes into account explicitly the fact that expression profiles in temporal experiments may be serially correlated and uses a model-based, Bayesian procedure to identify the best grouping of the gene expression data in an automated way.

CAGED implements a Bayesian clustering method designed to handle temporal experiments and subsuming standard independent experiments as a special case.

::DEVELOPER

Marco Ramoni & Paola Sebastiani

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

CAGED

:: MORE INFORMATION

You will need a password to use this program. Please email sebas@bu.edu for a password.

WPDB 2.2 – The Protein Data Bank Through Windows

WPDB 2.2

:: DESCRIPTION

WPDB is a Microsoft Windows based program for browsing and interrogating native and derived structural features of biological macromolecules using data obtained from the Protein Data Bank (PDB). Major features of WPDB are a 20-fold compression of PDB files and query and analysis tools. The latter permit the geometric and sequence properties of structures to be analyzed individually or through comparative analysis. The object oriented software design provides a high level of interaction between display windows which facilitates information discovery.

WPDB is useful for detailed analysis of a single structure or comparative analysis of two or more structures. It comes with a variety of databases. Alternatively, you can build your own database from PDB files with the WPDB Loader (WPDBL).

WPDBL Used to build a WPDB database from PDB files for access by the WPDB program. Prebuilt databases of a complete PDB distribution are available already built in the ../ directory.

::DEVELOPER

Ilya Shindyalov and Phil Bourne

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

WPDB ; WPDBL

:: MORE INFORMATION

Those using WPDB should cite:
I.N.Shindyalov and P.E.Bourne J. App. Cryst. 1995, 28(6) 847-852. WPDB A PC-based Tool for Analyzing Protein Structure. [Postscript]

OligoArray 2.1.3 – Genome-scale Oligonucleotide Design for Microarrays

OligoArray 2.1.3

:: DESCRIPTION

OligoArray is a free software that computes gene specific oligonucleotides for genome-scale oligonucleotide microarray construction. Selection is based on three major criteria: oligonucleotide melting temperature, specificity to a single target, or at least to the shortest list of possible targets and the inability to fold to form a stable secondary structure at the hybridization temperature.

::DEVELOPER

Jean-Marie Rouillard

:: SCREENSHOTS

:: REQUIREMENTS

:: DOWNLOAD

OligoArray

:: MORE INFORMATION

Citation:

OligoArray 2.0: Design of oligonucleotide probes for DNA microarrays using a thermodynamic approach
Jean – Marie Rouillard, Michael Zuker and Erdogan Gulari , Nucleic Acids Research, 2003, Vol. 31, No. 12 3057-3062

Spotfinder v3.2.1 – Microarray Images Analysis & Gene Expression Quantification

Spotfinder v3.2.1

:: DESCRIPTION

Spotfinder is an image processing program created at The Institute for Genomic Research (J. Craig Venter Institute now) for analysis of the image files generated in microarray expression studies. Spotfinder uses a fast and reproducible algorithm to identify the spots on array and provide quantitation of expression levels.

Image analysis is a crucial step in the microarray process. TIGR Spotfinder was designed for the rapid, reproducible and computer-aided analysis of microarray images and the quantification of gene expression. TIGR Spotfinder reads paired 16-bit or 8-bit TIFF image files generated by most microarray scanners. Semi-automatic grid construction defines the areas of the slide where spots are expected. Automatic and manual grid adjustments help to ensure that each rectangular grid cell is centered on a spot. Two available segmentation methods (histogram and Otsu) define the boundaries between each spot and the surrounding local background. Spot intensities are calculated as an integral of non-saturated pixels, although other options including spot median and mean values are available. Local background subtraction for each reported value is applied by default but can be disabled. The calculated intensities, medians, and means along with each spot position on the array, spot area, background values, and quality control flags are written to a MEV file or the database. Reusable grid geometry files and automatic grid adjustment allow user to analyze large quantities of images in a consistent and efficient manner. To complement the automated methods, particularly in noisy areas of the slide, the user may manually identify or discard spots. Quality control views allow the user to assess systematic biases in the data.

::DEVELOPER

J. Craig Venter Institute

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows/Mac OS X on Intel chips/Mac OS X on PowerPC chips/Linux

:: DOWNLOAD

Spotfinder v3.2.1 for Win ; for Mac OS X on Intel chips ; for Mac OS X on PowerPC chips ; for Linux ; Manual ; Source Code ; Training Documents

:: MORE INFORMATION

Referencing Spotfinder

Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J. TM4: a free, open-source system for microarray data management and analysis. Biotechniques. 2003 Feb;34(2):374-8.

MADAM 4.1 – MicroArray DAta Manager

MADAM 4.1

:: DESCRIPTION

Madam (Microarray Data Manager) is a suite of tools used to upload,download, and display a plethora of microarray data to and from a database management system (MySql). Working as an interface for the MySql, Madam allows scientists and researchers to manage their microarray data efficiently to meet the requirement of experiment annotation and data mining.

Madam implemented in Java, facilitates the entry of data into a relational database. MADAM guides users through the microarray process from RNA procurement to data analysis, offering intelligent forms to simplify the tracking of experimental parameters and results that are essential for the interpretation of expression results in downstream analyses. Canned reports provide information on RNA samples, studies, slide maps and other pertinent data and a general SQL query window allows freeform access to the underlying database.

MADAM also serves as a platform for launching other data entry and management tools. Through the use of these integrated modules, users can view and score PCR plates, design experiments and studies, and track laboratory materials. Although not yet supported, MADAM is being adapted to read and write MAGE-ML, the XML data exchange format being developed by an international consortium of leading public databases and microarray research centers. A MAGE-ML version of MADAM should be available by the end of this year and will facilitate submission of microarray data to public repositories such as Array Express and GEO.

::DEVELOPER

J. Craig Venter Institute

:: SCREENSHOTS

:: REQUIREMENTS

:: DOWNLOAD

MADAM v4.0 for Win ; MADAM 4.1 for Linux ; Source Code ;  Manual ; Training Documents

:: MORE INFORMATION

Referencing MADAM

Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J. TM4: a free, open-source system for microarray data management and analysis. Biotechniques. 2003 Feb;34(2):374-8.