Off-Spotteris a tool that when presented with a candidate gRNA and a PAM, quickly and exhaustively identifies all genomic sites that are identical or nearly-identical to the gRNA and also satisfy the PAM constraint.
the Computational Medicine Center at the Sidney Kimmel Medical College of Thomas Jefferson University
:: MORE INFORMATION
“Off-Spotter: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs“,
Venetia Pliatsika and Isidore Rigoutsos.
Biol. Direct. 2015;10 :4. doi: 10.1186/s13062-015-0035-z.