The NGSQC pipeline provides a set of novel quality control measures for quickly detecting a wide variety of quality issues in deep sequencing data derived from two dimensional surfaces, regardless of the assay technology used. It also enables researchers to determine whether sequencing data related to their most interesting biological discoveries are caused by sequencing quality issues. NGSQC can help to ensure that biological conclusions, in particular those based on relatively rare sequences, are not caused by low quality sequencing.
Microarray Lab, University of Michigan
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BMC Genomics. 2010 Dec 2;11 Suppl 4:S7. doi: 10.1186/1471-2164-11-S4-S7.
NGSQC: cross-platform quality analysis pipeline for deep sequencing data.
Dai M1, Thompson RC, Maher C, Contreras-Galindo R, Kaplan MH, Markovitz DM, Omenn G, Meng F.