Jul 122014
 

NASTIseq 1.0

:: DESCRIPTION

NASTIseq is a software package for the identiffcation of cis-Natural Antisense Transcripts (cis-NAT) using strand speciffc RNA-seq data. The method is based on the observation that strand speciffc RNAseq sometimes generate reads from the unexpected strand. We designed a probabilistic model which estimate the strand speciffcity and account for it using a binomial model.

::DEVELOPER

Uwe Ohler’s Research Group

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / Windows
  • R
  • DESeq
  • ROCR

:: DOWNLOAD

 NASTIseq

:: MORE INFORMATION

Citation

Song Li, Louisa Liberman, Neelanjan Mukherjee, Philip N. Benfey, Uwe Ohler. (2013).
Integrated detection of natural antisense transcripts using strand-specific RNA sequencing data.
Genome Res. 2013 Oct;23(10):1730-9. doi: 10.1101/gr.149310.112.

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