Mutate is a program for the emulation of the so called fluctuation test. It is developed to teach a virtual laboratory class for intermediate-level undergraduate students in Biology. The key idea is to train students in some multidisciplinary aspects of current genetics as sequence databases, DNA mutations and hypothesis testing, while introducing the fluctuation test. During the practice the students should download some sequences, e.g the fhuA gene responsible for membrane receptor of phage T1 in E. coli, introduce some stop codon mutations and design a fluctuation test, using adequate parameter values for the number of bacteria, the number of plates, the mutation rate and the number of experimental replicates, to obtain data for distinguishing between preadaptative (spontaneous) and direct (induced) mutation hypotheses.
- Linux/windows /MacOsX
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Teaching the fluctuation test in silico by using mutate: A program to distinguish between the adaptive and spontaneous mutation hypotheses
Biochemistry and Molecular Biology Education Volume 40, Issue 4, pages 277–283, July/August 2012