Lucy has been used for several years to clean sequence data from automated DNA sequencers prior to sequence assembly and other downstream uses. The quality trimming portion of lucy makes use of phred quality scores, such as those produced by many automated sequencers based on the Sanger sequencing method. As such, lucy’s quality trimming may not be appropriate for sequence data produced by some of the new “next-generation” sequencers.
- Linux / Windows /Mac OsX
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1. LUCY2: an interactive DNA sequence quality trimming and vector removal tool
Bioinformatics Advance Access published on May 6, 2004
Song Li and Hui-Hsien Chou Bioinformatics, Nov 2004; 20: 2865-2866.
2. DNA sequence quality trimming and vector removal
Hui-Hsien Chou and Michael H. Holmes.Bioinformatics. 2001. 17(12):1093-1104.