LaneRuler will identify lanes in a gel image. The lanes on such gels may not be straight and parallel due to various reasons, and these deviations must be accounted for in order to accurately size the restriction fragments in each lane. In order to meet the high throughput requirements by the projects at the British Columbia Cancer Agency Genome Sciences Center (GSC), the software has capability to verify and correct its results automatically, and prompting for user inspection only for extremely abnormal cases. In validation testing using Bacterial Artificial Chromosome (BAC) clones, the automatic lane tracking results gave restriction fragment sizing results that are comparable to those from manually supervised lane tracking results, achieving sensitivity and specificity of restriction fragment identification exceeding 95%. The current conception of the program is able to successfully process 96% of the gels with no human intervention.
- Windows / Mac / Linux
:: MORE INFORMATION
Wong, R.T.F.; Flibotte, S.; Corbett, R.; Saeedi, P.; Jones, S.J.M.; Marra, M.A.; Schein, J.E.; Birol, I.; (2010 )
LaneRuler: Automated Lane Tracking for DNA Electrophoresis Gel Images
Automation Science and Engineering, IEEE Transactions Volume: 7 Issue:3 706 – 708