KLab PCR is a tool that implements two algorithms to quantify the starting template in a PCR reaction. The first is a modified Ct strategy that assumes an ideal efficiency and is calculated for reference. The second employs a parametric fitting approach to construct a non-linear approximation to the reaction efficiency that is regressed backwards to establish a peak efficiency. The implementation can use data from a single PCR reaction, or from several reactions where the same primers are used yielding a consensus of peak efficiency.
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Platts AE, Johnson GD, Linnemann AK, Krawetz SA.;
Real-time PCR quantification using a variable reaction efficiency model.
Anal Biochem. 2008 Sep 15;380(2);315-22