In Vitro Sim II is a program that simulates an in vitro evolution experiment in which random mutations are introduced into amplicons during PCR amplification of a template DNA coding sequence by the error-prone thermostable DNA polymerase Mutazyme II? marketed by Stratagene as part of the GeneMorph II? kit.
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Barlow-Hall in vitro Evolution Protocol
Barry G. Hall and Miriam Barlow
Nature Precedings hdl:10101/npre.2008.1772.1