FretTrace is the implementation of a maximum likelihood based theory. Single molecule Fluorescence Resonance Energy Transfer (FRET) experiments are a powerful and versatile tool for studying conformational motions of single biomolecules at a millisecond time scale. Typically, the small number of recorded photons limits the achieved time resolution.
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G. F. Schroder and H. Grubmuller,
Maximum likelihood trajectories from single molecule fluorescence resonance energy transfer experiments
J. Chem. Phys. 119: 9920-9924 (2003)