Ebbie is a semi-automated smRNA cloning data processing algorithm, which initially searches for any substring within a DNA sequencing text file, which is flanked by two constant strings. The substring, also termed smRNA or insert, is stored in a MySQL and BlastN database. These inserts are then compared using BlastN to locally installed databases allowing the rapid comparison of the insert to both the growing smRNA database and to other static sequence databases.
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H Alexander Ebhardt , Kay C Wiese and Peter J Unrau
Ebbie: automated analysis and storage of small RNA cloning data using a dynamic web server
BMC Bioinformatics 2006, 7:185doi:10.1186/1471-2105-7-185