The E-zyme software has been developed in the KEGG project as a means to better computerize the information about enzymatic reactions. It currently performs an automatic assignment of EC numbers based on the RDM system for reaction classification.
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E-zyme: predicting potential EC numbers from the chemical transformation pattern of substrate-product pairs.
Yamanishi Y, Hattori M, Kotera M, Goto S, Kanehisa M.
Bioinformatics. 2009 Jun 15;25(12):i179-86. doi: 10.1093/bioinformatics/btp223.