DPROT is a bi-layer cascade Support Vector Machine (SVM). This method involves the use of bi-layer. The first layer SVM classifiers was trained and optimized with different individual protein sequence features like amino acid composition, dipeptide composition (occurrences of the possible pairs of i and i+1 amino acid residues), higher order dipeptide composition (pairs of i and i+2 residues) and PSSM (Position Specific Scoring Matrix) composition and secondary structure composition.
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Amino Acids. 2008 Oct;35(3):599-605. doi: 10.1007/s00726-008-0085-y. Epub 2008 Apr 19.
DPROT: prediction of disordered proteins using evolutionary information.
Sethi D1, Garg A, Raghava GP.