DART-PCR (Data Analysis for Real‐Time PCR) provides a simple means of analysing real-time PCR data from raw flurescence data. This allows an automatic calculation of amplification kinetics, as well as performing the subsequent calculations for relative quantification and calculation of assay variability. Amplification efficiencies are also tested to dtect anomalus samples within groups (outlayers) and differences between experimatal groups (amplification equivalence).
Stuart N. Peirson, Jason N. Butler and Russell G. Foster
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Stuart N. Peirson, Jason N. Butler and Russell G. Foster (2003)
Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis
Nucl. Acids Res. (2003) 31 (14): e73