CloneAssistant is a standalone software, which can automatically design the cloning primers for users with full consideration of the sequence information of vectors and genes, cloning strategies, the principles of primer design, reading frame, position effects, and enzymatic reaction conditions. It can be used for restriction map analysis, ORF (open reading frame) finding, sequence alignment and complementary analysis, translation, restriction enzyme and universal buffer query, and isocaudamer analysis.
BIS @ Zhejiang University
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J Biotechnol. 2010 Nov;150(3):294-8. doi: 10.1016/j.jbiotec.2010.09.952.
CloneAssistant 1.0: a stand-alone software for automated cloning primer design.
Shao C, Meng Y, Lv S, Zhong W, Wang Z, Chen M.