BiSearch is a primer-design algorithm for DNA sequences. It may be used for both bisulfite converted as well as for original not modified sequences. You can search various genomes with the designed primers to avoid non-specific PCR products by our fast ePCR method. This is especially recommended when primers are designed to amplify the highly redundant bisulfite treated sequences.
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Arányi T, Váradi A, Simon I and Tusnády GE (2006)
The BiSearch web server.
BMC Bioinformatics 7, 431.