Jan 062012
 

Bareback 1.0

:: DESCRIPTION

Barebackis a tool to shuffle Illumina image files with low complexity sequence at the start of all sequences to the end.Bareback-processing allows the user to ‘move’ the raw images acquired by the Illumina Genome Analyser IIx from initial cycles with a very biased sequence composition to the end of the run. For samples with very biased initial sequences (such as restriction enzyme sites) and/or very high cluster densities this procedure can allow to a dramatic increase in sequence yield. This approach also works very well for custom-made multiplexing applications.

:: DEVELOPER

Babraham Bioinformatics

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux/ Windows /Mac OsX
  • Perl

:: DOWNLOAD

 Bareback

:: MORE INFORMATION

Citation

PLoS One. 2011 Jan 28;6(1):e16607.
Large scale loss of data in low-diversity illumina sequencing libraries can be recovered by deferred cluster calling.
Krueger F, Andrews SR, Osborne CS.

229 views

Sorry, the comment form is closed at this time.