ASC (Analysis of Sequence Counts) borrows information across sequences to establish prior distribution of sample variation, so that biological variation can be accounted for even when replicates are not available. Compared current approaches that simply tests for equality of proportions in two samples, ASC is less biased towards highly expressed sequences and can identify more genes with a greater log fold change at lower overall abundance.
- Linux / Windows / MacOsX
- R package
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BMC Bioinformatics. 2010 Nov 16;11:564. doi: 10.1186/1471-2105-11-564.
Empirical bayes analysis of sequencing-based transcriptional profiling without replicates.
Wu Z, Jenkins BD, Rynearson TA, Dyhrman ST, Saito MA, Mercier M, Whitney LP.